Genetic diversity analysis of tree peony germplasm using iPBS markers

Genetic diversity analysis of tree peony germplasm using iPBS markers.

We examined the genetic diversity of 10 wild species (populations) and 55 varieties of tree peony using inter-primer binding site (iPBS) markers. From a total of 36 iPBS primers, 16 were selected based on polymorphic amplification. The number of bands amplified by each primer ranged from 9 to 19, with an average of 12.88 bands per primer. The length of bands ranged from 100 to 2000 bp, concentr...

Development of Pineapple Microsatellite Markers and Germplasm Genetic Diversity Analysis

Two methods were used to develop pineapple microsatellite markers. Genomic library-based SSR development: using selectively amplified microsatellite assay, 86 sequences were generated from pineapple genomic library. 91 (96.8%) of the 94 Simple Sequence Repeat (SSR) loci were dinucleotide repeats (39 AC/GT repeats and 52 GA/TC repeats, accounting for 42.9% and 57.1%, resp.), and the other three ...

Analysis of genetic diversity among wild bermudagrass germplasm from southwest China using SSR markers.

Fifty-five wild accessions of bermudagrass (Cynodon dactylon) were collected from southwest China (Sichuan, Chongqing, Yunnan, Guizhou, and Tibet), and their genetic diversity was analyzed using simple sequence repeat markers. A total of 267 polymorphic bands were detected with 18 primer combinations. The genetic similarity among the accessions ranged from 0.688 to 0.894 with an average of 0.79...

Genetic Diversity Analysis in Grape (Vitis vinifera L) Germplasm using Microsatellite Markers

Genetic diversity analysis of recombinant inbred lines of rice (Oryza sativa L.) using microsatellite markers

Estimation of genetic diversity is an important factor in germplasm conservation and characterization. In rice breeding programs, genetic diversity information on specific regions of genome can be very useful for the application of marker assisted selection (MAS) and for gene mapping. A total of 152 rice lines were considered for breeding programs using microsatellites (SSR) technique. The tota...